Open AccessMajor outer membrane proteins in the phytopathogenic bacteria Erwinia carotovora subsp. carotovora and subsp. atroseptica
Author(s) -
Saarilahti Hannu T.,
Palva E.Tapio
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01540.x
Subject(s) - erwinia , porin , biology , bacteria , microbiology and biotechnology , bacterial outer membrane , pectobacterium carotovorum , escherichia coli , enterobacteriaceae , trypsin , biochemistry , pathogen , enzyme , gene , genetics
The major outer membrane proteins from two subspecies of the phytopathogenic bacterium Erwinia carotovora were characterized. Protein patterns of E. carotovora ssp. carotovora isolates showed considerable heterogeneity in respect to both minor and major proteins, whereas isolates of E. carotovora ssp. atroseptica were almost identical. All isolates studied contained only two major proteins with M r values from 36 500 to 39 500. One of these major proteins resembled the OmpA protein of Escherichia coli : it was heat‐modifiable and partially trypsin‐sensitive, producing an M r 24 000 tryptic fragment. The other major protein species showed properties characteristic of porin proteins: it was resistant to trypsin and could be isolated as a protein‐peptidoglycan complex.