Open AccessConjugative co‐transfer of lactose and bacteriophage resistance plasmids from Streptococcus cremoris UC653
Author(s) -
Baumgartner Andreas,
Murphy Maeve,
Daly Charles,
Fitzgerald Gerald F.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01534.x
Subject(s) - plasmid , bacteriophage , microbiology and biotechnology , streptococcaceae , biology , lactose , bacteria , streptococcus , escherichia coli , food science , genetics , dna , gene , antibiotics
When conjugative transfer of lactose‐fermenting ability (Lac) was observed between Streptococcus cremoris UC653 (donor) and S. lactis MG1363 Sm (recipient), 70% of the Lac + transconjugants had acquired total resistance to phage 712 and propagated phage C2 at a lower efficiency and with a reduced plaque size. Plasmid analysis of transconjugants combined with curing experiments showed that the Lac and phage resistance markers were associated with plasmids of 26 and 50 MDa, respectively. Some transconjugants contained a large plasmid of either 77 or 83 MDa which coded for both Lac and phage resistance. The phage resistance mechanism did not act at the adsorption stage and was not affected by incubation at 37°C.