Open AccessLocalization of O9‐lipopolysaccharide within the Escherichia coli K29 capsule
Author(s) -
Bayer M.E.,
Weed D.,
Haberer S.,
Bayer M.H.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01521.x
Subject(s) - escherichia coli , lipopolysaccharide , bacterial outer membrane , capsule , bacterial capsule , secretion , microbiology and biotechnology , inner membrane , biology , antigen , enterobacteriaceae , chemistry , membrane , biochemistry , immunology , gene , botany , virulence
Lipopolysaccharides which are masked by the presence of a large capsule in many Gram‐negative organisms such as Escherichia coli K29, can be localized in ultrathin sections of fixed and Lowicryl‐embedded cells. The ‘post‐embedding‐immuno‐labeling’ procedure revealed a layered organization of the antigens, with the O‐9 specific lipopolysaccharide at the outer membrane's surface and the extensive K29 polysaccharide coat encapsulating the organism.