Open AccessA new peptidoglycan hydrolase in Streptococcus pneumoniae
Author(s) -
SańchezPuelles J.M.,
Ronda Concepción,
García E.,
Méndez E.,
García J.L.,
López R.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01520.x
Subject(s) - autolysin , amidase , peptidoglycan , streptococcus pneumoniae , hydrolase , lytic cycle , microbiology and biotechnology , mutant , lysin , muramidase , biology , biochemistry , enzyme , gene , escherichia coli , antibiotics , genetics , virus , bacteriophage
The use of a mutant of Streptococcus pneumoniae deleted in the lytA gene coding for the N‐acetyl‐muramyl‐ l ‐alanine amidase, and therefore devoid of any amidase, has allowed the identification of a new murein hydrolase activity in this bacterium. This enzyme (or enzymes) acted as an autolysin when the cultures were grown at 30°C. Our results strongly suggest that the new lytic activity corresponds to one or more glycosidases.