Open AccessCoordinate increase of isopenicillin N synthetase, isopenicillin N epimerase and deacetoxycephalosporin C synthetase in a high cephalosporin‐producing mutant of Acremonium chrysogenum and simultaneous loss of the three enzymes in a non‐producing mutant
Author(s) -
Ramos F.R.,
LópezNieto M.J.,
Martín J.F.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01513.x
Subject(s) - mutant , enzyme , biochemistry , cephalosporin c , cephalosporin , acremonium , biology , chemistry , gene , antibiotics , botany
An early blocked mutant in cephalosporin biosynthesis ( Acremonium chrysogenum N 2 ) had simultaneously lost 3 enzymes of the cephalosporin biosynthetic pathway (isopenicillin N synthetase, isopenicillin N epimerase and deacetoxycephalosporin C synthetase) and accumulated the tripeptide α‐aminoadipyl‐cysteinyl‐valine. An overproducing mutant ( A. chrysogenum C‐10) showed a 2‐fold increase in the same 3 enzymes throughout fermentation, with respect to the low‐producing strain A. chrysogenum CW‐19. These results suggest that expression of the genes coding for cephalosporin biosynthetic enzymes is altered in a coordinate form in these mutants.