Open AccessThermophilic degradation of cellulose by a triculture of Clostridium thermocellum, Methanobacterium sp. and Methanosarcina MP
Author(s) -
Smiti N.,
Ollivier B.,
Garcia J.L.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01506.x
Subject(s) - clostridium thermocellum , methanobacterium , fermentation , chemistry , methanomicrobiales , clostridium , methanosarcina , cellulose , euryarchaeota , ethanol , methanosarcina barkeri , biochemistry , food science , microbiology and biotechnology , bacteria , biology , methanogenesis , archaea , cellulase , organic chemistry , methane , genetics , gene
The fermentation of cellulose at 55°C by different associations of the 3 bacteria Clostridium thermocellum, Methanobacterium sp. and Methanosarcina MP, was studied. C. thermocellum alone produced acetate, lactate, ethanol, H 2 and CO 2 . The co‐culture C. thermocellum‐Methanobacterium sp. produced more acetate and less ethanol than the monoculture of Clostridium . Methanosarcina MP used acetate only in the triculture including Methanobacterium sp. When methanol was added (5 mM) to the triculture, Methanosarcina MP had a shorter lag phase on acetate and degraded much more acetate. maximum methane production was 8.5 mmol CH 4 /g cellulose degraded.