Open AccessA method to transform the β‐lactam antibiotic producer Streptomyces wadayamensis
Author(s) -
Acebal C.,
Rubio V.,
Márquez G.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01503.x
Subject(s) - protoplast , transformation (genetics) , streptomyces , polyethylene glycol , streptomycetaceae , antibiotics , microbiology and biotechnology , lysozyme , bacteria , chemistry , actinomycetales , biology , biochemistry , genetics , gene
We have developed a transformation system for Streptomyces wadayamensis , a cephamycin C producer. 1−5 × 10 9 protoplasts can be obtained when late logarithmic phase cultures of this microorganisms are incubated with 10 mg/ml of lysozyme. Polyethylene glycol‐Ca 2+ ‐mediated transformation of these protoplasts yielded 10 6 transformants per μg of pIJ702 or pIJ365 DNA.