Open AccessNitrogenase in free‐living and symbiotic cyanobacteria: Immunoelectron microscopic localization
Author(s) -
Bergman Birgitta,
Lindblad Peter,
Rai Amar N.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01502.x
Subject(s) - heterocyst , nitrogenase , immunoelectron microscopy , cyanobacteria , biology , anabaena , botany , phycobilisome , nitrogen fixation , microbiology and biotechnology , bacteria , genetics , antibody
Nitrogenase (Fe‐protein) was localized in the free‐living cyanobacterium Anabaena cylindrica and in the cyanobionts of Cycas revoluta and Peltigera aphthosa , using colloidal gold as an immunocytochemical marker. The Fe‐protein was found to be evenly distributed throughout the heterocyst cytoplasma in A. cylindrica and in both the cyanobionts, including multiple heterocysts of the C. revoluta cyanobiont. No label was observed in the vegetative cells of free‐living A. cylindrica or of the cyanobionts, although the cyanobionts apparently live under microaerobic conditions.