Open AccessGlucose uptake and phosphorylating activities in two species of slow‐growing Rhizobium
Author(s) -
San Francisco Michael J.D.,
Jacobson Gary R.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01501.x
Subject(s) - phosphoenolpyruvate carboxykinase , rhizobium , pep group translocation , phosphorylation , biochemistry , biology , phosphotransferase , glucose uptake , enzyme , microbiology and biotechnology , insulin , gene
Glucose uptake and phosphorylating activities were studied in two strains of slow‐growing Rhizobium: Rhizobium japonicum (USDAI‐110) and cowpea Rhizobium (USDA3278). Cultured cells of both species actively took up glucose, and at least two systems appeared to be involved, whereas purified bacteroids of both species failed to accumulate glucose actively. In both cell types, glucose phosphorylation was ATP‐dependent, and no evidence was obtained for a phosphoenolpyruvate‐dependent glucose phosphotransferase system.