Open AccessA Klebsiella pneumoniae plasmid which codes for isocitrate dehydrogenase
Author(s) -
Tomás Juan M.,
Parés Ramón,
Somers Jackie M.,
Kay William W.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01495.x
Subject(s) - isocitrate dehydrogenase , isocitrate lyase , idh1 , plasmid , biology , klebsiella pneumoniae , dehydrogenase , biochemistry , enzyme , microbiology and biotechnology , gene , mutant , escherichia coli , glyoxylate cycle
A naturally occurring Klebsiella pneumoniae plasmid of 114 MDa was able to complement isocitrate dehydrogenase deficiencies in K. pneumoniae and Salmonella typhimurium . The plasmid encoded isocitrate dehydrogenase, which differed from that of S. typhimurium in the kinetic parameters for NADP and isocitrate, in its isoelectric point, and in its response to repression by cAMP. This is the first report of a naturally occurring plasmid encoding a Krebs cycle enzyme.