Open AccessRing dechlorination of deethylsimazine by hydrolases from Rhodococcus corallinus
Author(s) -
Cook Alasdair M.,
Hütter R.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01432.x
Subject(s) - rhodococcus , chemistry , amine gas treating , triazine , gel permeation chromatography , enzyme , chromatography , organic chemistry , biochemistry , polymer
Cell‐free extracts of Rhodococcus corallinus NRRL B‐15444R dechlorinated deethylsimazine (2‐chloro‐4‐ethylamino‐1,3,5‐triazine‐6‐amine) to N ‐ethylammeline (2‐amino‐4‐ethylamino‐1,3,5‐triazine‐6(5H)‐one) anaerobically, and, thus, presumably hydrolytically. Two enzyme fractions ( M r about 450 000 and 180 000) that dechlorinated deethylsimazine were detected after gel permeation chromatography of cell extracts.