Open AccessIsolation and microcalorimetric characterisation of glucose‐negative and pyruvate‐negative mutants of Mycoplasma mycoides subsp. mycoides
Author(s) -
Lee D.H.,
Miles R.J.,
Beezer A.E.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01421.x
Subject(s) - mycoplasma mycoides , mutant , strain (injury) , biochemistry , biology , fructose , mannose , microbiology and biotechnology , mycoplasma , anatomy , gene
A glucose‐negative and a pyruvate‐negative strain of Mycoplasma mycoides ssp. mycoides were isolated by their resistance to 3‐deoxy‐3‐fluoro‐ d ‐glucose and β‐fluoropyruvate, respectively. The ability of the mutants to metabolise various substrates was investigated microcalorimetrically. Results suggest that both mutants are transport mutants. The pyruvate‐negative mutant was unable to metabolise exogenous lactate. The kinetics of N ‐acetylglucosamine and fructose metabolism by the glucose‐negative mutant were similar to those of the parent strain; glucosamine and mannose, however, were not metabolised, and it is suggested that their transport in the parent strain involves glucose‐specific uptake component(s).