Open AccessSterol biosynthesis via lanosterol by the trypanosomid Crithidia fasciculata
Author(s) -
Raederstorff Daniel,
Rohmer Michel
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01418.x
Subject(s) - lanosterol , crithidia fasciculata , cycloartenol , ergosterol , sterol , biochemistry , biosynthesis , biology , algae , protozoa , crithidia , microbiology and biotechnology , botany , cholesterol , enzyme
Incorporation of [1‐ 14 C]acetate by the trypanosomid Crithidia fasciculata showed that ergosterol biosynthesis occurs de novo in this protozoon, via lanosterol and 31‐norlanosterol. No cycloartenol could be detected, indicating that this biosynthesis pathway is rather similar to those of other non‐photosynthetic organisms (animals, fungi). From the point of view of sterol biosynthesis, C. fasciculata is not related to other ergosterol‐synthesising protozoa, such as the hitherto examined phytoflagellates and soil amoebae, which synthesise their sterols via cycloartenol, like photosynthetic organisms (plants, algae).