Open AccessNucleic acid hybridisation with a probe specific for 3 aminoglycoside acetyltransferase type IV: A survey of resistance to apramycin and gentamicin in animal strains of Escherichia coli
Author(s) -
ChaslusDancla Elisabeth,
Gerbaud Guy,
Lafont JeanPierre,
Martel JeanLouis,
Courvalin Patrice
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01417.x
Subject(s) - escherichia coli , microbiology and biotechnology , gentamicin , aminoglycoside , biology , acetyltransferase , bacteria , antibiotic resistance , antibiotics , gene , acetylation , biochemistry , genetics
Resistance to apramycin due to production of a 3‐aminoglycoside acetyltransferase type IV (AAC(3)IV) has recently been detected among Gram‐negative bacteria isolated in France from bovine clinical samples. 24 apramycin‐resistant Escherichia coli strains isolated over the country, and epidemiologically unrelated, were studied by colony hybridization using an intragenic probe specific for AAC(3)IV. The results obtained indicated that the structural gene for the acetyltransferase was present in all the isolates tested and in the corresponding apramycin‐resistant transconjugants. This observation demonstrates that resistance to apramycin by acetylation of the antibiotic has spread very rapidly in bovine Gram‐negative bacteria.