Open AccessCovalent modification of proteins in Escherichia coli growing anaerobically with nitrate as electron acceptor
Author(s) -
Quentmeier Armin,
Antranikian Garabed
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01410.x
Subject(s) - escherichia coli , phosphodiester bond , biochemistry , covalent bond , electron acceptor , chemistry , isoelectric point , alkaline phosphatase , gel electrophoresis , phosphatase , enzyme , organic chemistry , rna , gene
Escherichia coli cells were labeled with [ 32 P]orthophosphate under anaerobic conditions using nitrate as electron acceptor. By 2‐dimensional gel electrophoresis, 20 protein species were found to be radioactively labeled; their isoelectric points and molecular masses were determined. Treating the labeled extract with alkaline phosphatase revealed evidence for the presence of 9 proteins modified by phosphorylation. Treatment with snake venom phosphodiesterase also indicated that 2 protein species in E. coli were modified by phosphate‐containing compounds which were bound to the protein by a phosphodiester bond.