Open AccessA simple method for the detection of two types of thiaminase‐producing colonies
Author(s) -
Abe Mitsuko,
Nishimune Takahiro,
Ito Shinichi,
Kimoto Mitsuaki,
Hayashi Ryoji
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01391.x
Subject(s) - clostridia , agar plate , enzyme , agar , bacillus (shape) , substrate (aquarium) , microbiology and biotechnology , biology , chemistry , biochemistry , bacteria , ecology , genetics
A plate method which can detect two types of thiaminase‐producing colonies was developed. After growing bacterial colonies on a plate with good thiaminase production for 2–4 days, soft agar was overlayed which contained the substrate thiamin, and substituting base (pyridoxine) for type I enzyme, and a buffer of appropriate pH for each type of enzyme. The plate was incubated at 37°C for 1–2 h for Bacillus thiaminolyticus and clostridia, and at 60°C for 2 h for Bacillus aneurinolyticus . Good results were obtained for laboratory strains of average thiaminase activity.