Open AccessResonance Raman scattering by bacterial spores
Author(s) -
Shibata Hirofumi,
Taniguchi Junsuke,
Komuro Masahito,
Ohe Mayumi,
Yamashita Shinsuke,
Tani Isamu
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01349.x
Subject(s) - bacillus megaterium , spore , raman spectroscopy , dipicolinic acid , raman scattering , endospore , bacillus (shape) , resonance (particle physics) , chemistry , bacteria , nuclear magnetic resonance , biology , analytical chemistry (journal) , microbiology and biotechnology , chromatography , optics , physics , atomic physics , genetics
The laser Raman spectra of lyophilized spores of 5 species (6 strains) of Bacillus were examined. Under the experimental conditions employed, only Bacillus megaterium species showed 3 intense Raman bands at 1515, 1157 and 1007 cm −1 . Spores of other species, despite their high content of dipicolinic acid, did not show distinct Raman signals. The strong, scattering bands at 1515 and 1157 cm −1 in the spectra of spores of B. megaterium may be attributable to conjugated double‐bond systems, probably of membrane‐associated carotenoids. Their high intensities are due to resonance enhancement.