Open AccessImmunological comparison of microbial TPP‐dependent non‐oxidative α‐keto acid decarboxylases
Author(s) -
Barrowman Margaret M.,
Harnett William,
Scott Alan J.,
Fewson Charles A.,
Kusel John R.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01348.x
Subject(s) - acinetobacter calcoaceticus , biochemistry , pseudomonas , biology , pseudomonas putida , enzyme , microbiology and biotechnology , chemistry , bacteria , genetics , acinetobacter , antibiotics
Antigenic, and hence possible evolutionary, relationships amongst various TPP‐dependent non‐oxidative α‐keto acid decarboxylases were determined by the Ouchterlony double diffusion method and by measuring the degree of antibody‐induced enzyme inhibition. The results show that: (a) phenylglyoxylate decarboxylases of various wild‐type strains of Acinetobacter calcoaceticus are antigenically indistinguishable; (b) there seems to be no antigenic cross‐reactivity between the phenylglyoxylate decarboxylase of A. calcoaceticus and of Pseudomonas aeruginosa or Pseudomonas putida ; and (c) no antigenic homology can be detected amongst phenylglyoxylate decarboxylase and phenylpyruvate decarboxylase of A. calcoaceticus and pyruvate decarboxylase of brewers' yeast.