Open AccessNetropsin stimulates the formation of an extracellular proteinase and suppresses protein turnover in sporulating Bacillus megaterium
Author(s) -
Kučerová Helena,
Strnadová Marie,
Vinter Vladimír,
Graba Peter,
Chaloupka Jiří
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01341.x
Subject(s) - bacillus megaterium , extracellular , protein turnover , chemistry , bacillus (shape) , microbiology and biotechnology , biochemistry , biology , protein biosynthesis , bacteria , genetics
Netropsin stimulated the rate of synthesis of an extracellular metalloproteinase in Bacillus megaterium incubated in a sporulation medium. The antibiotic delayed but did not suppress the decrease in the ability to synthesize the proteinase occurring at later sporulation stages. Netropsin also stimulated the synthesis of the proteinase when added to a growing culture; it inhibited the increase of protein turnover which was switched on between the 2nd and 3rd hour in the sporulating population. No refractile spores were developed during 6 h at 35°C in the antibiotic‐treated culture. In the control 60% of sporulating cells were observed under similar conditions.