Open AccessNucleotide sequence of a plasmid‐encoded hemolysin determinant and its comparison with a corresponding chromosomal hemolysin sequence
Author(s) -
Hess J.,
Wels W.,
Vogel M.,
Goebel W.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01339.x
Subject(s) - genetics , hemolysin , plasmid , biology , nucleic acid sequence , homology (biology) , gene , sequence (biology) , insertion sequence , escherichia coli , sequence analysis , consensus sequence , peptide sequence , genome , transposable element , virulence
The complete sequence of the plasmid pHly152‐encoded hemolysin ( hly ) determinant of Escherichia coli is presented and compared with a recently sequenced chromosomal hly determinant [1]. High sequence homology between the two hly determinants is observed withìn all four structural genes, hlyC, A, B and D , but little sequence similarities are found in the 3′‐ and 5′‐noncoding flanking regions. In addition, the noncoding region upstream of hlyC which carries the promoter for hlyC, A and B , was sequenced for several chromosomal hly determinants. The comparison of these sequences indicates three distinct classes of promoter regions which share common putative −10 and −35 boxes at roughly the same location relative to the start of hlyC .