Open AccessMetabolism of non‐motile obligately methylotrophic bacteria
Author(s) -
Trotsenko Y.A.,
Doroni.V.,
Govorukhi.I.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01290.x
Subject(s) - bacteria , metabolism , microbial metabolism , microbiology and biotechnology , biology , chemistry , biochemistry , genetics
A study was made of the enzymology of primary and intermediary pathways of C 1 metabolism in three strains of non‐motile obligately methylotrophic bacteria. Each uses a variant of the ribulosemonophosphate (RMP) cycle of formaldehyde fixation which involves the Entner‐Doudoroff route for hexose‐phosphate cleavage and transaldolase/transketolase mode of rearrangement. The organisms possess high levels of hexulose‐phosphate synthase and NAD(P)‐linked glucose‐6‐phosphate and 6‐phosphogluconate dehydrogenases. In addition they contain small activities of dye‐linked methanol and methylamine dehydrogenases, PMS‐ and NAD‐linked formaldehyde and formate dehydrogenases. This indicates cyclic rather than direct oxidation of formaldehyde derived from methanol or methylamine. The tricarboxylic acid cycle is defective in 2‐ketoglutarate dehydrogenase and the glyoxylate shunt is not operating because of the absence of malate synthase. Oxaloacetate is regenerated by (phosphoenol) pyruvate carboxylases. NH + 4 is assimilated mainly by glutamate dehydrogenase. The results show metabolic similarities between motile and non‐motile obligate methanol and methylamine utilizers.