Open AccessSecretion by Streptomyces lividans of a cloned Gram‐negative beta‐lactamase
Author(s) -
Ali Norryai A.,
Dale Jeremy W.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01286.x
Subject(s) - plasmid , biology , escherichia coli , shuttle vector , gene , microbiology and biotechnology , enterobacteriaceae , streptomyces , streptomycetaceae , bacteria , genetics , actinomycetales , vector (molecular biology) , recombinant dna
The OXA‐2 β‐lactamase gene was first found on a conjugative plasmid R46 from a clinical isolate of Salmonella typhimurium . To transfer the gene to Streptomyces lividans a shuttle vector was created by fusing an Escherichia coli plasmid carrying the OXA‐2 β‐lactamase gene with the S. lividans vector pIJ61. The OXA‐2 β‐lactamase gene was expressed in S. lividans , although with a much reduced efficiency; virtually all of the β‐lactamase activity was found in the culture supernatant. The identity of the enzyme was established by substrate specificity and isoelectric focusing.