Open AccessNucleotide sequence of the F plasmid gene srnB that promotes degradation of stable RNA in Escherichia coli
Author(s) -
Akimoto Shigeru,
Ono Kenji,
Ono Tsuneko,
Ohnishi Yoshinari
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01279.x
Subject(s) - plasmid , sequence (biology) , nucleotide , nucleic acid sequence , rna , degradation (telecommunications) , chemistry , escherichia coli , biology , microbiology and biotechnology , biochemistry , dna , gene , telecommunications , computer science
We determined the nucleotide sequence of a DNA fragment of the F plasmid containing the srnB gene responsible for promotion of stable RNA degradation in Escherichia coli . From the DNA sequence, a possible initiation codon (ATG) was preceded by a possible promoter about 100 bp upstream, and by a Shine‐Dalgarno sequence (GAGG) with proper spacing. The ATG was followed by the sequence of a putative polypeptide product and likely translation and transcription termination signals. A possible base‐paired stem and loop was detected between the promoter and the initiation codon. This region may participate in the regulation of srnB expression.