Open AccessEffects of lkyB mutations on the expression of ompF, ompC and lamB porin structural genes in Escherichia coli K‐12
Author(s) -
Lazzaroni Jean Claude,
FogniniLefebvre Nicole,
Portalier Raymond C.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01278.x
Subject(s) - lac operon , porin , bacterial outer membrane , operon , periplasmic space , escherichia coli , mutant , biology , gene , gene product , microbiology and biotechnology , structural gene , mutation , transcription (linguistics) , gene expression , genetics , linguistics , philosophy
Periplasmic‐leaky mutants of Escherichia coli K‐12 carrying a lkyB mutation had a reduced content of outer membrane proteins OmpF and LamB. The effect of the lkyB 207 mutation on the expression of ompF, ompC or lamB genes was analysed in strains carrying a ompF‐lacZ, ompC‐lacZ or lamB‐lacZ operon or gene fusion. It was shown that the lkyB 207 mutation exerted a 1.5‐ to 2‐fold effect on the transcription of ompF‐lacZ and ompC‐lacZ operon fusions, as well as on the expression of lamB‐lacZ gene fusions. The results suggested that the lkyB gene product exerted a positive control on LamB and OmpF protein levels but a negative control on the OmpC protein level.