Open AccessAntibiotic resistance caused by permeability changes of the archaebacterium Methanococcus vannielii
Author(s) -
Haas E.S.,
Hook L.A.,
Reeve J.N.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01268.x
Subject(s) - bacitracin , neomycin , thiostrepton , mutant , cell envelope , biology , microbiology and biotechnology , biochemistry , ribosome , chemistry , antibiotics , escherichia coli , gene , rna
Spontaneous mutants of the methane‐producing archaebacterium Methanococcus vannielii have been isolated which are resistant to bromoethane‐sulphonate, A2315‐A (a virginiamycin), neomycin and thiostrepton. Ultraviolet (UV) irradiation followed by growth in bacitracin led to the isolation of bacitracin‐resistant mutants. In vitro translation reactions using ribosomes prepared from neomycin or A2315‐A‐resistant mutants were sensitive to neomycin or A2315‐A indicating that the in vivo resistance of the mutants must reflect changes in the permeability of the M. vannielii proteinaceous cell envelope.