Open AccessTransposition of Tn 1831 to sym plasmids of Rhizobium leguminosarum and Rhizobium trifolii
Author(s) -
Pees Elly,
Wijffelman Carel,
Mulders Ine,
Brussel Anton A.N.,
Lugtenberg Ben J.J.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01265.x
Subject(s) - rhizobium leguminosarum , rhizobium , transposition (logic) , plasmid , biology , genetics , rhizobiaceae , bacteria , gene , symbiosis , philosophy , linguistics
All transposon‐induced symbiotic mutants of Rhizobium described so far have been obtained using Tn 5 , which codes for kanamycin resistance (Km R ). To enable genetic complementation studies, we tried to find an effective transposon carrying another resistance marker. We report here a method for the apparent random transposition in Rhizobium of Tn 1831 , which codes for resistance against spectinomycin (Sp), streptomycin (Sm) and mercury chloride. When the suicide plasmid pMP12 (RP4::Tn 1831 , Km::Mu) was transferred to Rhizobium , in almost all cases the exconjugants harbour a deleted transfer‐deficient R plasmid. From this deleted R plasmid transposition occurred to self‐transmissible Sym‐plasmids of R. leguminosarum and R. trifolii . Using this method a number of Tn 1831 ‐induced symbiotic mutants of pRL1JI were isolated.