Open AccessIncorporation of diaminopimelic acid into Escherichia coli membranes
Author(s) -
Bayer M.E.,
Bayer M.H.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01229.x
Subject(s) - diaminopimelic acid , membrane , escherichia coli , bacterial outer membrane , inner membrane , biophysics , cell division , chemistry , biochemistry , cell membrane , biology , microbiology and biotechnology , cell , cell wall , peptidoglycan , gene
Pulse‐labeled, cell division‐inhibited Escherichia coli incorporate [ 3 H]diamino pimelic acid (DAP) into membrane fractions of light (= inner membrane) and intermediate densities and transfer the DAP rapidly into heavy membrane fractions (= outer membrane). The intermediate density fraction which has been shown to contain markers for membrane adhesion sites appears to serve as intermediary in the translocation.