
13 C‐NMR studies on the influence of pH and nitrogen source on polyol pool formation in Aspergillus nidulans
Author(s) -
Dijkema Cor,
Rijcken Robert Pels,
Kester Harry C.M.,
Visser Jaap
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01225.x
Subject(s) - mannitol , aspergillus nidulans , mycelium , polyol , sucrose , arabitol , chemistry , ammonium , nitrogen , biochemistry , nitrate , sorbitol , aspergillus , ammonium nitrate , food science , nuclear chemistry , biology , fermentation , organic chemistry , botany , mutant , polyurethane , xylitol , gene
The composition of the polyol pools in Aspergillus nidulans mycelium during active growth on sucrose depends strongly on pH. At pH 2.5, only mannitol is present. A comparison between nitrate‐ and ammonium‐grown cultures shows stimulation of the arabitol content with nitrate a former nitrogen source. When starved mycelium is incubated either with natural‐abundance or 13 C‐enriched glucose, label appears rapidly in mannitol and arabitol, regardless of the nitrogen source or the pH used.