Open AccessMolecular cloning and expression of the Streptococcus lactis tagatose 1,6‐bisphosphate aldolase gene in Escherichia coli
Author(s) -
Limsowtin Gaetan K.Y.,
Crow Vaughan L.,
Pearce Lindsay E.
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01216.x
Subject(s) - plasmid , escherichia coli , ecori , aldolase a , pbr322 , molecular cloning , biology , microbiology and biotechnology , recombinant dna , cloning (programming) , lactococcus lactis , gene , genetics , enzyme , biochemistry , bacteria , gene expression , computer science , programming language , lactic acid
A 4.4 kb Eco RI DNA fragment of the Streptococcus lactis H 1 plasmid pDI1 was cloned into the Escherichia coli plasmid pACYC 184. The recombinant plasmid expressed d ‐tagatose 1,6‐bisphosphate aldolase activity in E. coli . Enzyme activity was at the same level as in the original S. lactis host but was not repressed by glucose.