Open AccessDNA methylase activities in a Neisseria gonorrhoeae extract
Author(s) -
Prere MarieFrançoise,
Fayet Olivier
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01207.x
Subject(s) - neisseria gonorrhoeae , haeiii , restriction enzyme , bamhi , biology , dna , escherichia coli , genetics , microbiology and biotechnology , cytosine , gene , genotype , restriction fragment length polymorphism
Resistance of Neisseria gonorrhoeae DNA to cleavage by various restriction endonucleases suggests the existence of modification enzymes which protect the corresponding recognition sequences. We indeed found methylase activities in N. gonorrhoeae extracts. These activities lead to the methylation of adenine and cytosine residues in bacteriophage λ DNA and DNA from an Escherichia coli Dam − strain. They also result in partial protection of λ DNA to cleavage by the restriction endonucleases Hae II, Hae III, Bam HI and, Sac II.