Open AccessPurification and some properties of Clostridium botulinum type AB toxin
Author(s) -
Sakaguchi Genji,
Sakaguchi Sumiko,
Kozaki Shunji,
Takahashi Motohide
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01205.x
Subject(s) - toxin , clostridium botulinum , trypsinization , chemistry , microbiology and biotechnology , clostridium difficile toxin a , biology , biochemistry , trypsin , enzyme , clostridium difficile , antibiotics
The progenitor toxin of Clostridium botulinum type AB was purified; both large‐sized (L) and medium‐sized (M) toxins were found. The toxicity of M toxin increased by about 10‐fold upon trypsinization; the increase was due mostly to type B toxin and a little to type A toxin. M toxin appeared to consist of one molecule each of toxic and nontoxic components. The activated toxic component was made up of four fragments, A‐H‐ and L‐chains and B‐H‐ and L‐chains. AB toxin may be a mixture of A and B toxins.