Open AccessMutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl‐tRNA synthetase
Author(s) -
Kiener Andreas,
Rechsteiner Thomas,
Leisinger Thomas
Publication year - 1986
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1986.tb01203.x
Subject(s) - isoleucine , mutant , transfer rna , enzyme , biochemistry , strain (injury) , wild type , biology , chemistry , stereochemistry , microbiology and biotechnology , amino acid , leucine , rna , gene , anatomy
Growth of Methanobacterium thermoautotrophicum strain Marburg was strongly inhibited by 2 μg/ml of pseudomonic acid. This inhibition was relieved by 10 mM l ‐isoleucine. The susceptibility of a pseudomonic acid‐resistant mutant was 50 times lower than in the wild type. Isoleucyl‐tRNA synthetase activity in cell extracts from the wild type was strongly inhibited by pseudomonic acid whereas the corresponding enzyme in mutant extracts was only marginally inhibited by the antibiotic.