Open AccessEvidence for the presence of lipopolysaccharide in Treponema phagedenis (biotype Reiterii) but not in Treponema pallidum (Nichols)
Author(s) -
Bailey M.J.,
Penn C.W.,
Cockayne A.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01650.x
Subject(s) - treponema , heterologous , microbiology and biotechnology , antiserum , treponema denticola , bacteria , biology , staining , protease , homologous chromosome , silver stain , lipopolysaccharide , virology , syphilis , enzyme , biochemistry , antibody , gene , genetics , immunology , porphyromonas gingivalis , human immunodeficiency virus (hiv)
Immunoblotting profiles of whole or protease‐K‐digested organisms with homologous antisera demonstrated the presence of a characteristic ladder pattern of smooth LPS in Treponema phagedenis . Periodic acid silver staining of SDS‐PAGE gels confirmed these findings. However, when heterologous or homologous serum was reacted with Treponema pallidum , no such pattern or cross‐reactions were observed. The significance of apparent absence of LPS in T. pallidum is discussed.