Open AccessLysis induced by uncoupling agents in Myxococcus coralloides D
Author(s) -
FernandezVivas A.,
Arias J.M.,
Montoya E.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01646.x
Subject(s) - lysis , sodium azide , dinitrophenol , valinomycin , biochemistry , biology , glycerol , microbiology and biotechnology , myxococcus xanthus , chemistry , membrane , mutant , gene
When sodium azide was added to cultures of Myxococcus coralloides D a rapid loss in turbidity was observed. The lysis occurred irrespective of the culture age. If the azide was added to cultures which had been division‐inhibited with puromycin, lysis was also induced. Other uncoupling agents (2,4‐dinitrophenol, methyltriphenylphosphonium bromide and N , N ′‐dicyclohexylcarbodiimide) were effective to induce lysis, but not the ionophores gramicidin D or valinomycin. Energizing the membrane by the addition of glycerol, glucose or ascorbate to prelytic cultures was a means of preventing the lytic events.