Open AccessHydrolysis of iodothyronine sulfates by sulfatase activity of anaerobic bacteria from the rat intestinal flora
Author(s) -
Herder W.W.,
Hazenberg M.P.,
Otten M.H.,
PennockSchröder A.M.,
Visser T.J.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01643.x
Subject(s) - bacteria , microbiology and biotechnology , anaerobic bacteria , biochemistry , hydrolysis , fermentation , biology , sulfate , triiodothyronine , eubacterium , chemistry , hormone , genetics , organic chemistry
2 Obligately anaerobic bacteria isolated from rat cecal flora have previously been shown to possess sulfatase activity towards 3,3′‐diiodothyronine sulfate [5]. These strains have now been tested for their ability to hydrolyze the sulfate conjugates of other iodothyronines, including the thyroid hormones thyroxine and 3,3′,5‐triiodothyronine. In anaerobic incubations at 37°C with approximately 10 7 bacteria per ml, variable amounts of the conjugated substrates, ranging from 15–90%, were hydrolysed in 24 h. These results showed a potent iodothyronine sulfate hydrolysing capacity of rat intestinal microflora. The strains were characterized by carbohydrate fermentation tests. One strain belonged to the genus Lactobacillus , the other strain probably to Eubacterium or Lachnospira .