Open AccessIsolation and characterization of a plasmid, pSLG3, from Streptomyces lavendulae‐grasserius
Author(s) -
Tichý P.,
Moskalenko L.N.,
Smrčková I.,
Krumphanzl V.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01640.x
Subject(s) - isolation (microbiology) , plasmid , biology , microbiology and biotechnology , streptomycetaceae , streptomyces , actinomycetales , genetics , bacteria , dna
A new, high copy number conjugative plasmid pSLG3 (10.9 kb) was isolated from vegetative mycelium of Streptomyces lavendulae‐grasserius RIA746. The sensitivity of pSLG3 DNA to 9 restriction endonucleases was tested and the positions of the unique Bgl II and Pst I target site, 3 Kpn I target sites and 5 Pvu II target sites were mapped. The unique Bgl II target site was localized outside pSLG3 essential region and was used for the construction of recombinant plasmid pSR1, composed of pSLG3 and pIJ350 Streptomyces DNAs.