Open AccessA fungal lectin and its apparent receptors on a nematode surface
Author(s) -
Borrebaeck Carl A.K.,
Mattiasson Bo,
NordbringHertz Birgit
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01633.x
Subject(s) - lectin , nematode , receptor , microbiology and biotechnology , biology , chemistry , immunology , biochemistry , ecology
A lectin was isolated from the nematode‐trapping fungus Arthrobotrys oligospora . This carbohydrate‐binding protein was developmentally regulated and was found only on trap‐bearing mycelia. The lectin receptor on the surface of the nematode Panagrellus redivivus has, furthermore, been investigated using homogenates from whole nematodes or nematode cuticle. The ability of macromolecules from nematodes, fractionated according to M r and lectin affinities, to inhibit the capture of nematodes by A. oligospora was tested using an inhibition assay, based on a simple dialysis membrane technique. One major lectin‐binding glycoprotein, with apparent M r of 65 000, was isolated from the nematode cuticle.