Open AccessSequence‐specific endonuclease from the transformable cyanobacterium Anacystis nidulans R2
Author(s) -
Gallagher Margaret L.,
Burke William F.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01618.x
Subject(s) - endonuclease , cleave , plasmid , enzyme , restriction enzyme , strain (injury) , recognition sequence , biology , bacteria , sequence (biology) , microbiology and biotechnology , biochemistry , cyanobacteria , bacillaceae , chemistry , dna , genetics , bacillus subtilis , anatomy
Extracts of the transformable cyanobacterial strain Anacystis nidulans R2 were analyzed for the presence of restriction endonuclease. One enzyme, Ani I, was found and determined to be sequence‐specific on the basis of its ability to cleave several Bacillus plasmids at a limited number of sites. The activity of this enzyme is significantly reduced in extracts prepared from cell cultures grown at 38°C.