Open AccessThe role of mitochondria in the uptake of methyltriphenylphosphonium ion by Saccharomyces cerevisiae
Author(s) -
Midgley M.,
Thompson C.L.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01617.x
Subject(s) - cytoplasm , mitochondrion , saccharomyces cerevisiae , transporter , limiting , yeast , biology , membrane , microbiology and biotechnology , biophysics , ion transporter , inner mitochondrial membrane , membrane potential , biochemistry , inner membrane , chemistry , gene , mechanical engineering , engineering
The uptake of a membrane potential (Δψ) probe by strain D27 of Saccharomyces cerevisiae has been shown to have properties consistent with entry occurring by means of a non‐mediated process rather than the thiamine transporter. Experiments in which the mitochondrial membrane was selectively depolarised indicated that the probe used partitioned into mitochondria thus limiting its usefulness for quantitative measurements of Δψ across the cytoplasmic membrane.