Open AccessProduction of monoclonal antibodies against Clostridium botulinum type E derivative toxin
Author(s) -
Kamata Yohichi,
Kozaki Shunji,
Nagai Takafumi,
Sakaguchi Genji
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01616.x
Subject(s) - monoclonal antibody , polyclonal antibodies , clostridium botulinum , toxin , antibody , titer , chemistry , microbiology and biotechnology , monoclonal , antiserum , biology , immunology
Five monoclonal antibodies (MCA; E–8–2, 9–1, 11–2, 12–4, and 13–1) against Clostridium botulinum type E derivative toxin were prepared. Their ELISA titers were higher than or equivalent to that of conventional polyclonal antibody. Three of them (E‐8–2, 12–4, and 13–1) possessed the neutralizing activity comparable to that of polyclonal antibody. The results of binding‐competition experiments indicated that the monoclonal antibodies bound to different sites on the type E toxin molecule. Immunoblotting analyses demonstrated that E‐8–2, 9–1, and 11–2 react to fragment I (heavy chain) of the toxin. By use of these monoclonal antibodies, it may be possible to scrutinize the structure‐function relationship of botulinum toxins and cross reactions between type E and F toxins.