Open AccessThe methionine sulphoxide reductase activity of the yeast dimethyl sulphoxide reductase system
Author(s) -
Gibson Richard M.,
Large Peter J.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01572.x
Subject(s) - methionine , reductase , enzyme , chemistry , yeast , biochemistry , saccharomyces cerevisiae , substrate (aquarium) , stereochemistry , biology , amino acid , ecology
Glycyl‐ l ‐methionine sulphoxide and N‐acetyl‐ l ‐methionine sulphoxide were less effective inhibitors of the dimethyl sulphoxide (DMSO) reductase activity of Saccharomyces cerevisiae NCYC240 than was l ‐methionine (±)‐sulphoxide. Methionine sulphoxide reductase and DMSO reductase activities from crude extracts co‐purified over five purification steps and the two activities eluted from columns in exactly the same fractions. Both activities were sensitive to the same inhibitors. It was concluded that: (1) the DMSO reductase activity of S. cerevisiae is a property of a methionine sulphoxide reductase different from that of Black et al. [J. Biol. Chem. 235 (1960) 2910–2916], and (2) free methionine sulphoxide rather than peptidyl methionine sulphoxide is probably the enzyme's true substrate.