Open AccessCytotoxicity of two proteins isolated from Bacillus thuringiensis var. israelensis crystals to insect and mammalian cell lines
Author(s) -
Chilcott C.N.,
Kalmakoff J.,
Pillai J.S.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01570.x
Subject(s) - bacillus thuringiensis , cytotoxicity , cytotoxic t cell , aedes aegypti , spodoptera , biology , microbiology and biotechnology , cell culture , viability assay , biochemistry , cell , larva , botany , in vitro , bacteria , recombinant dna , gene , genetics
Two toxic proteins (proteins A and B) were isolated from Bacillus thuringiensis var. israelensis crystals using ion‐exchange chromatography. A cell cytotoxicity assay based on the uptake of [ 3 H]uridine by tissue culture cells following exposure to the toxic proteins, was used to measure the viability of the cells. Proteins A and B were cytotoxic to Aedes aegypti and MA104 cells. Protein B was also cytotoxic to Spodoptera frugiperda cells.