Open AccessPlasma membrane structures of Saccharomyces cerevisiae and Candida albicans revealed by freeze‐fracturing before and after treatment with filipin
Author(s) -
Takeo Kanji
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01568.x
Subject(s) - filipin , ergosterol , membrane , candida albicans , saccharomyces cerevisiae , biophysics , hypha , chemistry , cell membrane , microbiology and biotechnology , yeast , biology , biochemistry
Plasma membrane structures of Saccharomyces cerevisiae and Candida albicans during growth were studied by means of freeze‐fracturing before and after filipin treatment. Undifferentiated regions of the plasma membrane were severely deformed by filipin, indicating the existence of a high level of ergosterol. The plasma membrane of small buds was mildly deformed by filipin, which suggested the existence of a low level of ergosterol. The bottom part of invaginations and the plasma membrane of the neck between the mother cell and the bud usually lacked filipin‐induced deformations. Constraints existed in these regions which might restrict the ability of filipin to deform the membrane.