Open AccessThe RNAs of two viruses of the Nudaurelia β family share little homology and have no terminal poly(A) tracts
Author(s) -
King Linda A.,
Moore Norman F.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01562.x
Subject(s) - complementary dna , rna , biology , homology (biology) , microbiology and biotechnology , primer (cosmetics) , virus , heterologous , genome , virology , genetics , gene , chemistry , organic chemistry
cDNA‐RNA liquid hybridization analysis was used to compare the RNA sequence homology between two members of the Nudaurelia β virus family, Trichoplusia ni virus ( T.ni V) and Dasychira pudibunda virus ( D.p V). Heterologous hybridization experiments demonstrated that these viruses shared little sequence homology. Using oligo(dT) chromatography and oligo(dT) 12–18 as a primer for cDNA synthesis it was shown that neither T.ni V nor D.p V RNA genomes possess a poly(A) tract at the 3′ end.