Open AccessEffect of monoclonal antibodies on the adherence of Bordetella pertussis to Vero cells
Author(s) -
Gorringe A.R.,
Ashworth L.A.E.,
Irons L.I.,
Robinson A.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01556.x
Subject(s) - bordetella pertussis , vero cell , monoclonal antibody , microbiology and biotechnology , virology , pertactin , biology , antibody , pertussis toxin , immunology , bacteria , g protein , biochemistry , virus , receptor , genetics
An in vitro assay involving the adherence of Bordetella pertussis to Vero cells was used to quantify the inhibitory effects of monoclonal antibodies (McAb) on adherence. McAbs to agglutinogens (fimbriae), filamentous haemagglutinin (FHA), lymphocytosis promoting factor (LPF) and to an X‐mode specific outer membrane protein were tested. X‐mode cells adhered to the Vero cells to a greater extent than C‐mode cells. McAb to agglutinogen 2 inhibited the adherence of B. pertussis bearing homologous agglutinogen. McAbs to LPF, FHA, agglutinogen 3 and X‐mode specific outer membrane protein also partially inhibited the adherence of B. pertussis to Vero cells.