Open AccessEffect of NaCl concentration on the synthesis of membrane phospholipid in a halophilic bacterium
Author(s) -
Hara Hisako,
Masui Masamiki
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01160.x
Subject(s) - phosphatidylglycerol , phospholipid , tris , ethanolamine , glycerol , chemistry , chromatography , phosphate , membrane , halophile , phosphatidic acid , betaine , phosphate buffered saline , nuclear chemistry , bacteria , biochemistry , biology , phosphatidylcholine , genetics
The effect of NaCl concentration on phospholipid synthesis was investigated in the resting cells of Pseudomonas halosaccharolytica . These cells were suspended in Tris‐HCl buffer, pH 7.8, containing 0.5 M, 1.0 M or 3.5 M NaCl and then pulse‐chased with various 14 C‐labelled precursors of phospholipids, such as serine, acetate, glycerol and glycerol 3‐phosphate. Similar results were obtained in all these precursors: the radioactivities incorporated into phosphatidyl ethanolamine decrease with increasing NaCl concentration in Tris‐HCl buffer, while those incorporated into phosphatidylglycerol remained constant between 0.5 M and 3.5 M NaCl.