Open AccessIsolation and characterization of Azotobacter chroococcum from the roots of Zea mays
Author(s) -
MartinezToledo M.V.,
GonzalezLopez J.,
Rubia T.,
RamosCormenzana A.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01149.x
Subject(s) - azotobacter chroococcum , zea mays , isolation (microbiology) , azotobacter , biology , botany , agronomy , bacteria , microbiology and biotechnology , genetics
Bacteria showing rapid growth on a nitrogenfree medium and acetylene‐reducing activity were isolated from maize roots collected from agricultural soils in Spain. The isolates were Gram‐negative motile rods and were identified as Azotobacter chroococcum . Acetylene‐reducing activity and microbial counts were determined on root segments from 7‐ and 30‐day‐old plants. Rates obtained were in the range of 0.0053–0.848 nmol C 2 H 2 · g −1 · h −1 . Root populations were 1.4–6.0 × 10 4 micro‐organisms · g −1 . These results showed that there was an association between A. chroococcum strains and roots of maize planted in some Spanish soils.