Open AccessCloning and molecular genetic analysis of the frd operon encoding the fumarate reductase of Proteus vulgaris
Author(s) -
Honoré Nadine,
Cole Stewart T.
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01117.x
Subject(s) - biology , proteus vulgaris , operon , escherichia coli , genetics , gene , cloning (programming) , molecular cloning , fumarate reductase , restriction enzyme , restriction map , dna , microbiology and biotechnology , mutant , chromosome , genomic dna , positional cloning , plasmid , peptide sequence , computer science , programming language
The frd operon encoding the fumarate reductase of Proteus vulgaris has been cloned and a restriction map of the flanking chromosomal region established. Although the cloned genes complement an Escherichia coli frdA mutant, the overall arrangement of the P. vulgaris chromosome near the frd operon is very different. None of the well‐characterised markers linked to the E. coli frd genes, such as ampC or mop , could be detected by genetic studies or DNA‐DNA hybridisation.