Open AccessLipopolysaccharides of three classes of supersensitive mutants of Salmonella typhimurium
Author(s) -
Sukupolvi Soila,
Helander Ilkka M.,
Hukari Ritva,
Vaara Martti,
Mäkelä P.Helena
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01107.x
Subject(s) - heptose , chemistry , ethylenediaminetetraacetic acid , mutant , bacterial outer membrane , biochemistry , salmonella , lipopolysaccharide , divalent , gel electrophoresis , bacteria , polyacrylamide gel electrophoresis , escherichia coli , microbiology and biotechnology , biology , gene , organic chemistry , enzyme , genetics , chelation , endocrinology
The lipopolysaccharide (LPS) of 3 recently identified antibiotic‐supersensitive mutants of Salmonella typhimurium was analyzed and compared to the parent. The amounts of glucose, galactose, heptose, 2‐keto‐3‐deoxyoctonate, total phosphorus, total amino groups, and fatty acids were similar in all of the LPS preparations, and typical of the chemotype Rb 2 of the rfaJ parent. Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) did not reveal any differences between the mutants and the parent. Ethylenediaminetetraacetic acid and polycations, which partially release LPS from intact cells (presumably by replacing or removing divalent cations between molecules in the outer membrane), liberated similar amounts of [ 14 C]alactose‐labelled LPS from the mutants and the parent.