Open AccessA novel metabolic pathway for creatinine degradation in Pseudomonas putida 77
Author(s) -
Yamada Hideaki,
Shimizu Sakayu,
Kim Jong Min,
Shinmen Yoshifumi,
Sakai Takeshi
Publication year - 1985
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1985.tb01106.x
Subject(s) - sarcosine , pseudomonas putida , creatinine , creatine , chemistry , biochemistry , glycine , enzyme , amino acid
A novel metabolic pathway for creatinine, degradation of creatinine to glycine via N ‐methylhydantoin, N ‐carbamoylsarcosine and sarcosine in turn, was found to operate in Pseudomonas putida 77. N ‐Methylhydantoin, sarcosine and glycine were detected as metabolites from creatinine, and enzyme activities responsible for the degradation of creatinine, N ‐methylhydantoin, N ‐carbamoylsarcosine and sarcosine were observed in a crude cell extract, while neither creatinine amidohydrolase activity nor creatine were detected at all. It is suggested that creatinine was utilized as a source of both carbon and nitrogen at the same time, and that N ‐methylhydantoin was also utilized efficiently.